Changes

Glycogen Determination from Tissues

20 bytes added, 12:50, 24 June 2014
updated product number for amyloglucosidase
* Ethanol
* 50 mM Sodium Acetate, pH 4.8
* Amyloglucosidase 0.3 mg/mL in 50 mM Sodium Acetate. Stored in -80. (Sigma A7420-5MG)
* Glucose quantification kit (Wako Autokit Glucose Buffer Solution cat # 439-9091; for protocol see http://www.wakodiagnostics.com/pi/pi_autokit_glucose.pdf)
* Glucose standard solution (200 or 500 mg/dL; Wako)