906
edits
Changes
Added notes about testing transfection efficiency of PEI batches.
* Aliquot 500 uL to 1000 uL and store in -80C
* Thawed solution can be stored at 4C for up to 2 months, label a tube when thawed
==Optimization==
Each batch of PEI should be tested for efficiency by transfecting cells with GFP at a 1:1-1:6 DNA:PEI ratio and checking the number of fluorescent cells 1-2 days after transfection. Follow the protocol in [[Production of LentiCRISPR Viruses]] starting after the table and ending before collecting any media.
[[ Category: CRISPR ]]