915
edits
Changes
→Crosslinking, Lysis and Shearing of DNA
10. Collect the crude nuclear prep by centrifuging at 2,000 rpm at 4°C for 5 minutes.
11. Resuspend pellet to 1 ml with RIPA Buffer.[[(Do not vortex the tubes and try to avoid bubbles. Bubbles will cause popping and loss of samples during sonication]]).
12. Using the Sonics VibraCell Sonicator, sonicate each 1.0 ml ChIP sample on ice, in a cold room, at Power Output 5 watts 6 times for 30 seconds each
