Difference between revisions of "Preparation of Tail Samples (for Genotyping)"
From Bridges Lab Protocols
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==Protocol== | ==Protocol== | ||
| − | # Combine 100-200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tube or in a well of a 96 well PCR plage | + | # Combine 100-200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tube or in a well of a 96 well PCR plage (Proteinase K stock = 10mg/mL) |
# Incubate at 55 degrees (O/N) | # Incubate at 55 degrees (O/N) | ||
# Incubate at 85 degrees for 45 min | # Incubate at 85 degrees for 45 min | ||
Revision as of 17:18, 26 June 2013
PBND Solution: Tail Lysis Buffer
- 50mM KCl
- 10mM Tris HCl (pH~8.3)
- 0.1 mg/mL MgCl2, 6H20
- 0.1 mg/mL Gelatin
- 0.45% (NP-40) Jgepal
- 0.45% Tween 20
Protocol
- Combine 100-200uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppindorf tube or in a well of a 96 well PCR plage (Proteinase K stock = 10mg/mL)
- Incubate at 55 degrees (O/N)
- Incubate at 85 degrees for 45 min
- Run PCR
