Difference between revisions of "Main Page"
From Bridges Lab Protocols
Davebridges (Talk | contribs) m |
Davebridges (Talk | contribs) m (→Metabolic Measurements) |
||
| Line 46: | Line 46: | ||
*[[Glucose Tolerance Test]] | *[[Glucose Tolerance Test]] | ||
*[[Insulin Tolerance Test]] | *[[Insulin Tolerance Test]] | ||
| + | *[[Measuring Fasting Insulin]] | ||
==Figure Generation and Data Management== | ==Figure Generation and Data Management== | ||
*[[Generation of Figures in Illustrator]] | *[[Generation of Figures in Illustrator]] | ||
Revision as of 14:46, 16 June 2009
Contents
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Fugene Transfection of 293T/COS Cells
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
- Luciferase Assay
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
- Cesium Chloride Preparation of DNA
Protein Analysis
Protein Quantification
- Bradford Assay
- Quantification by Absorbance at 280nm
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
