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Created page with "*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul *These should be submitted in 1.5mL tubes *Go to https://client-seqcore.brcf.med.um..."
*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul
*These should be submitted in 1.5mL tubes
*Go to https://client-seqcore.brcf.med.umich.edu/login.cgi?exp=1
**PI login: Bridges
**Dave must add you to the lab under his account, he will give you your username and the password needed
*Typically we will select Sanger Sequencing (the only low throughput option)
*Select 'Submit samples/plates'
*Select the appropriate short-code from the drop down menu at the top of this page
*Generally choose 'One-at-a-time Sample Submission' unless you have a lot
*From here enter the following for each section:
**The sample name (the sample name that mean something to you regarding the sequence)
**No discounts
**Mini-Prep Plasmid
**Whatever vector you used for this plasmid
**Size of the insert you are sequencing
**Custom Primers
**Do not save
**Do not rush
*Save and repeat with your other samples
*Go to ... for locations and drop off times
*These should be submitted in 1.5mL tubes
*Go to https://client-seqcore.brcf.med.umich.edu/login.cgi?exp=1
**PI login: Bridges
**Dave must add you to the lab under his account, he will give you your username and the password needed
*Typically we will select Sanger Sequencing (the only low throughput option)
*Select 'Submit samples/plates'
*Select the appropriate short-code from the drop down menu at the top of this page
*Generally choose 'One-at-a-time Sample Submission' unless you have a lot
*From here enter the following for each section:
**The sample name (the sample name that mean something to you regarding the sequence)
**No discounts
**Mini-Prep Plasmid
**Whatever vector you used for this plasmid
**Size of the insert you are sequencing
**Custom Primers
**Do not save
**Do not rush
*Save and repeat with your other samples
*Go to ... for locations and drop off times