Difference between revisions of "Preparing and Sending Tissues to Histology Core"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (Added link to histology form) |
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#Harvest tissue from animal, place into cassette and submerge in 10% neutral buffered formalin for 24-48 hrs (depending on thickness of sample). | #Harvest tissue from animal, place into cassette and submerge in 10% neutral buffered formalin for 24-48 hrs (depending on thickness of sample). | ||
#Move cassettes into 70% ethanol for 24 hrs. Samples can be left in 70% for long term storage, if required. Make sure the storage container is sealed so that the ethanol does not evaporate and samples are submerged for the entire storage period. | #Move cassettes into 70% ethanol for 24 hrs. Samples can be left in 70% for long term storage, if required. Make sure the storage container is sealed so that the ethanol does not evaporate and samples are submerged for the entire storage period. | ||
| − | #Bring cassettes in a biohazard bag (with 70% EtOH) and completed request form (place in pocket of bag) to the UMCCC Tissue Core, information on location, phone number and hours of operation can be found here: https://www.pathology.med.umich.edu/giordano_lab/histology.htm. The form can be found [[Media: | + | #Bring cassettes in a biohazard bag (with 70% EtOH) and completed request form (place in pocket of bag) to the UMCCC Tissue Core, information on location, phone number and hours of operation can be found here: https://www.pathology.med.umich.edu/giordano_lab/histology.htm. The form can be found [[Media:UMCCC_Histology_Request_Form.pdf | here]] |
#Cassettes, bags and order request forms (must be copied, no link online) can be found in the lab in the large cabinet to the left of the sink. | #Cassettes, bags and order request forms (must be copied, no link online) can be found in the lab in the large cabinet to the left of the sink. | ||
Latest revision as of 13:36, 3 February 2017
- Harvest tissue from animal, place into cassette and submerge in 10% neutral buffered formalin for 24-48 hrs (depending on thickness of sample).
- Move cassettes into 70% ethanol for 24 hrs. Samples can be left in 70% for long term storage, if required. Make sure the storage container is sealed so that the ethanol does not evaporate and samples are submerged for the entire storage period.
- Bring cassettes in a biohazard bag (with 70% EtOH) and completed request form (place in pocket of bag) to the UMCCC Tissue Core, information on location, phone number and hours of operation can be found here: https://www.pathology.med.umich.edu/giordano_lab/histology.htm. The form can be found here
- Cassettes, bags and order request forms (must be copied, no link online) can be found in the lab in the large cabinet to the left of the sink.
