Difference between revisions of "Preparation of Tail Samples (for Genotyping)"
From Bridges Lab Protocols
m (→Protocol: Proteinase K) |
(→Protocol) |
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==Protocol== | ==Protocol== | ||
| − | # Combine | + | # Combine 100uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppendorf tube or in a well of a 96 well PCR plate (Proteinase K stock = 10mg/mL) |
| − | # Incubate at 55 degrees (O/N) | + | # Incubate at 55 degrees (16 hours - O/N) |
| − | # Incubate at 85 degrees for | + | # Incubate at 85 degrees for 60 min |
| − | # | + | # Hold at 4 degrees |
| + | |||
[[Category: Mouse Work]] | [[Category: Mouse Work]] | ||
Revision as of 18:24, 17 February 2016
PBND Solution: Tail Lysis Buffer
- 50mM KCl
- 10mM Tris HCl (pH~8.3)
- 0.1 mg/mL MgCl2, 6H20
- 0.1 mg/mL Gelatin
- 0.45% (NP-40) Jgepal
- 0.45% Tween 20
Protocol
- Combine 100uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppendorf tube or in a well of a 96 well PCR plate (Proteinase K stock = 10mg/mL)
- Incubate at 55 degrees (16 hours - O/N)
- Incubate at 85 degrees for 60 min
- Hold at 4 degrees
