MediaWiki API result
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{
"compare": {
"fromrevid": 1,
"torevid": 2,
"*": "<tr><td colspan=\"2\" class=\"diff-lineno\" id=\"mw-diff-left-l1\" >Line 1:</td>\n<td colspan=\"2\" class=\"diff-lineno\">Line 1:</td></tr>\n<tr><td class='diff-marker'>\u2212</td><td class='diff-deletedline'><div><del class=\"diffchange diffchange-inline\"><big>'''MediaWiki has been successfully installed</del>.<del class=\"diffchange diffchange-inline\">'''<</del>/<del class=\"diffchange diffchange-inline\">big></del></div></td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">Real Time qPCR</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">Materials</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">cDNA for templates</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">Qiashredder and RNEasy kits from Qiagen</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">Superscript Kit from Invitrogen</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">SyberGreen PCR Master Mix Applied Biosystems</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">96 well qPCR plate</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">Primers (Dilute to 0</ins>.<ins class=\"diffchange diffchange-inline\">22 uM mixture of fwd and rev)</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">Generate primers using http:</ins>/<ins class=\"diffchange diffchange-inline\">/pga.mgh.harvard.edu/primerbank/index.html</ins></div></td></tr>\n<tr><td class='diff-marker'> </td><td class='diff-context'></td><td class='diff-marker'> </td><td class='diff-context'></td></tr>\n<tr><td class='diff-marker'>\u2212</td><td class='diff-deletedline'><div><del class=\"diffchange diffchange-inline\">Consult the [http://meta</del>.<del class=\"diffchange diffchange-inline\">wikimedia</del>.<del class=\"diffchange diffchange-inline\">org/wiki/Help:Contents User's Guide] for information on </del>using the <del class=\"diffchange diffchange-inline\">wiki software</del>.</div></td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">Protocol</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">RNA Extraction</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">1</ins>.<ins class=\"diffchange diffchange-inline\">Use RNEasy kit with Qiashredder</ins>. <ins class=\"diffchange diffchange-inline\"> For a 12 well plate, use 350 uL of RLT (add 10 uL B-ME per 1 mL of RLT buffer).</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">2.Scrape cells and pass through Qiashredder column.  Do the optional DNAse step at step 5 </ins>using the <ins class=\"diffchange diffchange-inline\">RNAse free DNAse from Qiagen.</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">3</ins>.<ins class=\"diffchange diffchange-inline\">Store at -20 until RT reaction</ins></div></td></tr>\n<tr><td class='diff-marker'> </td><td class='diff-context'></td><td class='diff-marker'> </td><td class='diff-context'></td></tr>\n<tr><td class='diff-marker'>\u2212</td><td class='diff-deletedline'><div><del class=\"diffchange diffchange-inline\">== Getting started ==</del></div></td><td class='diff-marker'>+</td><td class='diff-addedline'><div> </div></td></tr>\n<tr><td class='diff-marker'>\u2212</td><td class='diff-deletedline'><div><del class=\"diffchange diffchange-inline\">* [http://www</del>.<del class=\"diffchange diffchange-inline\">mediawiki</del>.<del class=\"diffchange diffchange-inline\">org/wiki/Manual:Configuration_settings Configuration settings list]</del></div></td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">RT-PCR Reaction</ins></div></td></tr>\n<tr><td class='diff-marker'>\u2212</td><td class='diff-deletedline'><div><del class=\"diffchange diffchange-inline\">* [http://www</del>.<del class=\"diffchange diffchange-inline\">mediawiki</del>.<del class=\"diffchange diffchange-inline\">org/wiki/Manual:FAQ MediaWiki FAQ]</del></div></td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">1</ins>.<ins class=\"diffchange diffchange-inline\">Use 8 uL of RNA per RT reaction</ins>.</div></td></tr>\n<tr><td class='diff-marker'>\u2212</td><td class='diff-deletedline'><div><del class=\"diffchange diffchange-inline\">* [https://lists</del>.<del class=\"diffchange diffchange-inline\">wikimedia</del>.<del class=\"diffchange diffchange-inline\">org/mailman</del>/<del class=\"diffchange diffchange-inline\">listinfo/mediawiki-announce MediaWiki release mailing list]</del></div></td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">2</ins>.<ins class=\"diffchange diffchange-inline\">Use Superscript RT-PCR kit from Invitrogen, following manufacturers instructions</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">3</ins>.<ins class=\"diffchange diffchange-inline\">Store cDNA at -20 until use</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div> </div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">Plate Preparation</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">1</ins>.<ins class=\"diffchange diffchange-inline\">Prepare dilutions of primers</ins>. <ins class=\"diffchange diffchange-inline\"> Need 9 uL per well.  Book 3h on qPCR machine </ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">2.Get 96 well block and keep on rack.  Do not touch bottom of plate.</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">3.Add 1 uL template per well.  </ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">4.Add 9 uL primer per well</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">5.Using PCR strip and multichannel pipettor, add 10 uL Master mix to each well</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div> </div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\">References (Saltiel Lab)</ins></div></td></tr>\n<tr><td colspan=\"2\"> </td><td class='diff-marker'>+</td><td class='diff-addedline'><div><ins class=\"diffchange diffchange-inline\"><pubmed>18829989<</ins>/<ins class=\"diffchange diffchange-inline\">pubmed></ins></div></td></tr>\n"
}
}