MediaWiki API result
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{
"compare": {
"fromid": 1,
"fromrevid": 1,
"fromns": 0,
"fromtitle": "Main Page",
"toid": 2,
"torevid": 2,
"tons": 0,
"totitle": "Real Time PCR From Cell Culture",
"*": "<tr><td colspan=\"2\" class=\"diff-lineno\" id=\"mw-diff-left-l1\">Line 1:</td>\n<td colspan=\"2\" class=\"diff-lineno\">Line 1:</td></tr>\n<tr><td class=\"diff-marker\" data-marker=\"\u2212\"></td><td class=\"diff-deletedline diff-side-deleted\"><div><del class=\"diffchange diffchange-inline\"><big>'''MediaWiki has been successfully installed</del>.<del class=\"diffchange diffchange-inline\">'''<</del>/<del class=\"diffchange diffchange-inline\">big></del></div></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">Real Time qPCR</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">Materials</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">cDNA for templates</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">Qiashredder and RNEasy kits from Qiagen</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">Superscript Kit from Invitrogen</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">SyberGreen PCR Master Mix Applied Biosystems</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">96 well qPCR plate</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">Primers (Dilute to 0</ins>.<ins class=\"diffchange diffchange-inline\">22 uM mixture of fwd and rev)</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">Generate primers using http:</ins>/<ins class=\"diffchange diffchange-inline\">/pga.mgh.harvard.edu/primerbank/index.html</ins></div></td></tr>\n<tr><td class=\"diff-marker\"></td><td class=\"diff-context diff-side-deleted\"><br></td><td class=\"diff-marker\"></td><td class=\"diff-context diff-side-added\"><br></td></tr>\n<tr><td class=\"diff-marker\" data-marker=\"\u2212\"></td><td class=\"diff-deletedline diff-side-deleted\"><div><del class=\"diffchange diffchange-inline\">Consult the [http://meta</del>.<del class=\"diffchange diffchange-inline\">wikimedia</del>.<del class=\"diffchange diffchange-inline\">org/wiki/Help:Contents User's Guide] for information on </del>using the <del class=\"diffchange diffchange-inline\">wiki software</del>.</div></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">Protocol</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">RNA Extraction</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">1.Use RNEasy kit with Qiashredder.\u00a0 For a 12 well plate, use 350 uL of RLT (add 10 uL B-ME per 1 mL of RLT buffer).</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">2</ins>.<ins class=\"diffchange diffchange-inline\">Scrape cells and pass through Qiashredder column</ins>. <ins class=\"diffchange diffchange-inline\"> Do the optional DNAse step at step 5 </ins>using the <ins class=\"diffchange diffchange-inline\">RNAse free DNAse from Qiagen.</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">3</ins>.<ins class=\"diffchange diffchange-inline\">Store at -20 until RT reaction</ins></div></td></tr>\n<tr><td class=\"diff-marker\"></td><td class=\"diff-context diff-side-deleted\"><br></td><td class=\"diff-marker\"></td><td class=\"diff-context diff-side-added\"><br></td></tr>\n<tr><td class=\"diff-marker\" data-marker=\"\u2212\"></td><td class=\"diff-deletedline diff-side-deleted\"><div><del class=\"diffchange diffchange-inline\">== Getting started ==</del></div></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div>\u00a0</div></td></tr>\n<tr><td class=\"diff-marker\" data-marker=\"\u2212\"></td><td class=\"diff-deletedline diff-side-deleted\"><div><del class=\"diffchange diffchange-inline\">* [http://www</del>.<del class=\"diffchange diffchange-inline\">mediawiki</del>.<del class=\"diffchange diffchange-inline\">org/wiki/Manual:Configuration_settings Configuration settings list]</del></div></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">RT-PCR Reaction</ins></div></td></tr>\n<tr><td class=\"diff-marker\" data-marker=\"\u2212\"></td><td class=\"diff-deletedline diff-side-deleted\"><div><del class=\"diffchange diffchange-inline\">* [http://www</del>.<del class=\"diffchange diffchange-inline\">mediawiki</del>.<del class=\"diffchange diffchange-inline\">org/wiki/Manual:FAQ MediaWiki FAQ]</del></div></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">1.Use 8 uL of RNA per RT reaction.</ins></div></td></tr>\n<tr><td class=\"diff-marker\" data-marker=\"\u2212\"></td><td class=\"diff-deletedline diff-side-deleted\"><div><del class=\"diffchange diffchange-inline\">* [https://lists</del>.<del class=\"diffchange diffchange-inline\">wikimedia</del>.<del class=\"diffchange diffchange-inline\">org/mailman/listinfo</del>/<del class=\"diffchange diffchange-inline\">mediawiki-announce MediaWiki release mailing list]</del></div></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">2.Use Superscript RT-PCR kit from Invitrogen, following manufacturers instructions</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">3.Store cDNA at -20 until use</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div>\u00a0</div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">Plate Preparation</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">1.Prepare dilutions of primers.\u00a0 Need 9 uL per well.\u00a0 Book 3h on qPCR machine </ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">2.Get 96 well block and keep on rack</ins>. <ins class=\"diffchange diffchange-inline\"> Do not touch bottom of plate</ins>.</div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">3</ins>.<ins class=\"diffchange diffchange-inline\">Add 1 uL template per well</ins>. <ins class=\"diffchange diffchange-inline\"> </ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">4</ins>.<ins class=\"diffchange diffchange-inline\">Add 9 uL primer per well</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">5</ins>.<ins class=\"diffchange diffchange-inline\">Using PCR strip and multichannel pipettor, add 10 uL Master mix to each well</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div>\u00a0</div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\">References (Saltiel Lab)</ins></div></td></tr>\n<tr><td colspan=\"2\" class=\"diff-side-deleted\"></td><td class=\"diff-marker\" data-marker=\"+\"></td><td class=\"diff-addedline diff-side-added\"><div><ins class=\"diffchange diffchange-inline\"><pubmed>18829989<</ins>/<ins class=\"diffchange diffchange-inline\">pubmed></ins></div></td></tr>\n"
}
}